Coding
LovTAP-VP1

Part:BBa_K838000:Design

Designed by: Nicolas Gobet   Group: iGEM12_EPF-Lausanne   (2012-09-21)

Mammalian LovTAP-VP16


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 274
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 772
    Illegal XhoI site found at 817
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 274
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As you'll have noticed, this part contains an illegal XbaI site. A mutagenesis to introduce a silent mutation was planned, but the kit didn't arrive in time for the freeze. We'll submit the RFC10-compatble parts as soon as possible. In the meantime this part is useable as long as you don't need to use XbaI.


References